Considering patient preferences and regional variations in disease patterns, demographic characteristics, and healthcare approaches, the potential applicability of HUE ethnic medicine's conclusions to patients in other regions is assessed, taking into account clinical benefits, acceptable levels of risk, and patient acceptance criteria. The research conducted by the HUE team on ethnic medicine is meticulously structured to provide clear guidance for the development and research of novel ethnic remedies.
The quantity of a medication directly correlates to its safety and efficacy. A deep understanding of traditional Tibetan medicinal measuring units and their associated values is crucial for study. Surprise medical bills This study, leveraging Tibetan medical literature and modern experimental research, established the reference, nomenclature, and conversion factors for traditional Tibetan medicinal units. Meanwhile, the weight and volume of basic units were determined through extensive sampling and repeated measurements of reference samples. The traditional Tibetan medicine units of volume and weight were converted to their respective modern SI volume and weight unit counterparts, with a thorough validation of the findings' accuracy, dependability, and practicality. Furthermore, this investigation presented specific suggestions and reference points for crafting standardized units of weight and volume in Tibetan medicine. In the advancement of Tibetan medicine, guiding its processing, production, and clinical treatment is of considerable significance, as is promoting standardization and its standardized development.
Renowned within traditional Chinese medicine, Angong Niuhuang Pills, a time-honored formula, are esteemed as one of the 'three treasures of febrile diseases,' exhibiting proven effectiveness in addressing a range of ailments. In contrast, the existing literature lacks a bibliometric investigation of the development and future direction of Angong Niuhuang Pills research. Retrieving research articles pertaining to Angong Niuhuang Pills, published between 2000 and 2022, involved cross-referencing both CNKI and Web of Science databases, encompassing both Chinese and international publications. Key elements from the research articles were displayed visually using CiteSpace 61. Additionally, information extraction methods were applied to dissect the research status of Angong Niuhuang Pills, offering valuable insights into current research tendencies and prominent research areas. The data set comprises 460 Chinese articles and 41 English articles. The Beijing University of Chinese Medicine and Sun Yat-Sen University spearheaded the publication of the greatest number of research articles, both in Chinese and in English. A keyword analysis revealed that Chinese articles predominantly addressed cerebral hemorrhage, stroke, neurological function, coma, cerebral infarction, craniocerebral injury, and clinical applications, whereas English articles prioritized the mechanisms of cerebral ischemia, stroke, heavy metals, blood-brain barrier permeability, and oxidative stress. Future research efforts are likely to focus on the complex relationships among stroke, the blood-brain barrier, and oxidative stress. selleckchem Currently, the research endeavor surrounding Angong Niuhuang Pills is still in progress. Comprehensive research into the active components and mode of action of Angong Niuhuang Pills is essential, complemented by large-scale, randomized, controlled clinical trials for informed future development and application.
Bibliometrics were applied to thoroughly examine the focal points and the cutting-edge territories of gut microbiota research including traditional Chinese medicine (TCM), with the aim of inspiring novel approaches for forthcoming research in this specialized area. A search across CNKI, Wanfang, VIP, and Web of Science (WoS) yielded studies investigating gut microbiota in the context of traditional Chinese medicine (TCM), spanning the period from January 1, 2002, to December 31, 2021. Subsequent to data preparation and quality control, CiteSpace 58.R3 was used to graphically represent and investigate connections among authors, publications, and key terms. The study's materials included a considerable amount of 1,119 Chinese articles and 815 English articles. A surge in published articles, peaking in the 2019-2021 period, characterized this field's research activity. In the realm of Chinese and English publications, TAN Zhou-jin and DUAN Jin-ao were the authors who produced the largest volume of articles, respectively. These authors, whose publications topped both Chinese and English article lists, were central to this research field. The top five Chinese and English journals in this area had a significant impact on the international research landscape. Keyword analysis, focusing on high-frequency terms and clustering, highlighted four areas of concentrated research within the field: clinical trials and research on TCM's modulation of gut microbiota for disease treatment, the metabolic processes of Chinese medicines within the gut microbiota, and the impact of incorporating TCM into animal feed on animal growth performance and gut microbiota. A study focusing on gut microbiota structure in patients categorized by Traditional Chinese Medicine (TCM) syndromes, as well as exploring the utilization of TCM approaches in conjunction with probiotic/flora transplantation for disease management, could generate new perspectives on clinical diagnosis and traditional treatment methods. The future holds significant research potential in this area.
Atherosclerosis (AS) is a consequence of disturbed lipid metabolism, manifesting as lipid accumulation within the intima, subsequently triggering vascular fibrosis and calcification, culminating in the stiffening of the vascular wall. A substantial risk for the onset of AS is hyperlipidemia (HLP). Human hepatic carcinoma cell The 'nutrients return to the heart, fat accumulates in the channels' theory attributes the key pathogenic factor of AS to excess fat returning to the heart via the vascular system. Prolonged lipid buildup within the blood vessels, along with impaired blood flow, serve as the fundamental pathological mechanisms driving the onset of HLP and AS. The subsequent transformation of HLP into AS is marked by the manifestation of 'turbid phlegm and fat' and 'blood stasis' as pathological expressions. Didang Decoction (DDD) is a potent prescription that promotes blood circulation, removes blood stasis, resolves turbidity, decreases lipid levels, and opens blood vessels, consequently stimulating regeneration and exhibiting efficacy in the management of atherosclerotic diseases. High-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS) was used in this study to analyze the key blood components of DDD. Network pharmacology was then employed to investigate the potential targets and mechanisms through which DDD combats AS and HLP. The findings of the network pharmacology analysis were further corroborated by in vitro studies. From the DDD blood component pool, 231 samples were collected, including 157 which exhibited a composite score greater than 60. SwissTargetPrediction supplied 903 predicted targets. GeneCards, OMIM, and DisGeNET provided 279 disease targets. The intersection of these sets determined 79 potential target genes linked to DDD, AS, and HLP. Gene Ontology (GO) analysis suggested DDD's probable role in regulating biological processes such as cholesterol metabolism and inflammatory responses, and KEGG analysis demonstrated the presence of pathways like lipid and atherosclerosis, insulin resistance, chemo-carcinogenesis receptor activation, and AGE-RAGE signaling in diabetic complications. Cell culture experiments showed DDD to be capable of reducing free fatty acid-triggered lipid accumulation and cholesterol ester content in L02 cells, thereby enhancing cellular function. This effect may be mediated by increased expression of PPAR, LPL, PPARG, VEGFA, CETP, CYP1A1, and CYP3A4, and decreased expression of TNF-alpha and IL-6. A multi-component, multi-target, multi-pathway strategy employed by DDD may prove effective in preventing and treating both AS and HLP by impacting lipid metabolism, inflammatory responses, and apoptosis.
Using a network pharmacology and transcriptomics framework, the present study elucidated the mechanism of artesunate's action in treating bone destruction in experimental rheumatoid arthritis (RA). To determine differentially expressed genes (DEGs) related to artesunate's inhibition of osteoclast differentiation, transcriptome sequencing data were examined. GraphPad Prism 8 software was instrumental in plotting volcano maps, while the bioinformatics website was used to generate heat maps. By leveraging GeneCards and OMIM, insights into crucial targets of bone destruction were obtained within the context of rheumatoid arthritis. In rheumatoid arthritis (RA), the intersection of target genes associated with artesunate's impact on osteoclast differentiation and those responsible for bone destruction was identified by the Venny 21.0 program. These intersection genes were further assessed for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. By employing appropriate methods, the models of RANKL-induced osteoclast differentiation and collagen-induced arthritis (CIA) were constructed, culminating in the study. Artesunate's influence on bone destruction in rheumatoid arthritis (RA), both pharmacologically and mechanistically, was evaluated using quantitative real-time polymerase chain reaction (q-PCR), immunofluorescence, and immunohistochemistry. Artesunate intervention was applied to an in vitro osteoclast differentiation model prompted by RANKL stimulation. Transcriptome sequencing analysis identified 744 differentially expressed genes (DEGs) associated with artesunate's inhibition of osteoclast differentiation.